For a long time, this laboratory has used electron microscopy as a primary research method, to study the synaptic composition of the nervous system. This has given us considerable experience with serial-section EM, which is in routine use, as well as early experience with freeze-fracture methods; we are also now developing high-pressure freezing and freeze-substitution methods suitable to the nervous systems we analyse. Transmission EM has been recently upgraded with the recent acquisition of a fully digital Philips Tecnai 12 microscope. The embryology of the ascidian embryo has also been studied from scanning electron microscopy. These activities are all conducted in a fully equipped and integratedScientific Imaging Suite, located in the adjacent Department of Biology.