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Wholemount 3-D images of late tailbud embryos

  • embryos between 50-100% development. 40X/1.3 NA confocal image stacks at 2µm. Nuclear maps not cell maps- nuclei are not eucentric.
  • embryonic CNS at 126 (neuropore closing) -320 (pre-hatching) cell stages.
  • nuclei are stained with BOBO-3, colour-coded for their type and location in the neural tube’s cross section. Yellow: left lateral cell row; red: mitoses; blue: dorsal, ventral cells; green: neurohypophysis.
  • mitotic figures enable us to compare maps, identify progeny of dividing cells.
  • pigment cell nuclei (*).