- embryos between 50-100% development. 40X/1.3 NA confocal image stacks at 2µm.
Nuclear maps not cell maps- nuclei are not eucentric.
- embryonic CNS at 126 (neuropore closing) -320 (pre-hatching) cell stages.
- nuclei are stained with BOBO-3, colour-coded for their type and location in the neural tube’s cross section.
Yellow: left lateral cell row; red: mitoses; blue: dorsal, ventral cells; green: neurohypophysis.
- mitotic figures enable us to compare maps, identify progeny of dividing cells.
- pigment cell nuclei (*).