Example of a virtual brain reconstructed from confocal images stacks of the wholemounted adult brain of Drosophila, immunolabelled with anti-synaptotagmin (preparation by a former Research Associate,Xue-Jun Sun). It is sometimes hard to remember that such images are those of real brains, subject only to immunolabelling procedures, but that they are not real brains, because they image only the epitope of the particular antibody, leaving blank (in this case) cell bodies and axon tracts, which lack synaptotagmin.
Related image stacks have been created for the fly's brain at different
developmental stages, especially the brain of the late third-instar larva,
as well as for the larval brain of the ascidian tadpole larva at different
stages in embryogenesis.
X-axis rotation Quicktime: [